![]() Ultra-low-input RNA-Seq: Applies to total RNA input in the range of 10 pg - 5 ng.Library prep can be based on poly(A) mRNA enrichment (default), or rRNA depletion (at additional cost). Low-input RNA-Seq: Accommodates limited amounts of total RNA in the range of 5-100 ng.Can accommodate degraded RNA, such as those extracted from FFPE or laser capture microdissected samples. Targets both protein-coding genes and long noncoding RNAs. Total RNA-Seq: Starts with 100 ng to 1 ug total RNA.Aims to identify differentially expressed protein-coding genes. Prepares library from poly-A enriched mRNA species. mRNA-Seq: Starts with 100 ng to 1 ug high quality total RNA.NUSeq provides six types of RNA-seq services as detailed below: The more transcriptionally active a genomic region is, the more copies of RNA transcripts it produces, and the more RNA-Seq reads it generates. The total number of reads mapped to a particular genomic region represents the level of transcriptional activity in the region. After sequencing, the generated reads are mapped to the reference genome to identify their genomic origin. The libraries are then sequenced to generate millions of reads for each sample. RNA species need to undergo a sequencing library preparatory process prior to sequencing. This technology directly sequences RNA molecules in the transcriptome in order to determine their genes of origin and abundance. RNA sequencing, or RNA-Seq, is the latest technology to study the transcriptome, i.e., the full set of RNA transcripts as genome readouts in a cell or population of cells.
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